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Mouse IL-6 Quantikine ELISA Kit, 2 Plate (1 KT) M6

价    格:5394

产    地:美国更新时间:2019/7/15 13:41:34

品    牌:R&D型    号:M6000B

状    态:正常点击量:974

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北京百奥创新科技有限公司

联 系 人:吴女士

电     话:13366620421

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Mouse IL-6 Quantikine ELISA Kit, 2 Plate (1 KT) M6000B

  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    1.8 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse IL-6
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.



产品参数

现货促销价,数量1 ,售完为止


The Quantikine Mouse IL-6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-6. Results obtained using natural mouse IL-6 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-6.


Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, Heparin Plasma


Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020282227
Mean30872322985238
Standard Deviation23.48.11.87.518.1
CV%6.73.93.56.18.97.6


Recovery

The recovery of mouse IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.


Sample TypeAverage % RecoveryRange %
Cell Culture Supernates (n=5)11086-120
Heparin Plasma (n=6)10181-115
Serum (n=5)9984-113
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-6 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse IL-6 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-6
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
  • Long Name
    Interleukin 6
  • Entrez Gene IDs
    3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit);
  • Alternate Names
    B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A;
Related Research Areas
  • Adipocytokines

  • Cancer Biomarkers

  • Conventional/Classical Dendritic Cells

  • Cytokines and Receptors in Allergy and Asthma

  • Cytokines and Receptors in Angiogenesis

  • Cytokines and Receptors in Tfh Cell Differentiation and Function

  • Diabetes

  • gamma delta T Cells

  • Glucose Homeostasis

  • Helper T (Th) Cells

  • HIF Transcription Factors

  • IL-6 Family

  • Infectious Diseases

  • Inflammatory Mediators

  • Macrophage Activation Markers

  • Molecules Secreted by VSMC

  • Multiple Myeloma

  • Multiple Sclerosis and Myelin Related Molecules

  • Myeloid-derived Suppressor Cells (MDSC)

  • Neuroinflammation

  • Obesity

  • Plasmacytoid Dendritic Cells

  • T Cell Cytokine Signaling

  • Th17 Cells

  • Th22 Cells

  • Th9 Cells



产品介绍

  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL)
  • Sensitivity
    1.8 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
  • Specificity
    Natural and recombinant mouse IL-6
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.

  • Mouse IL-6 Quantikine ELISA Kit, 2 Plate (1 KT) M6000B
Product Summary
The Quantikine Mouse IL-6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-6. Results obtained using natural mouse IL-6 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-6.


Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, Heparin Plasma


Intra-Assay PrecisionInter-Assay Precision
Sample123123
n202020282227
Mean30872322985238
Standard Deviation23.48.11.87.518.1
CV%6.73.93.56.18.97.6


Recovery

The recovery of mouse IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.


Sample TypeAverage % RecoveryRange %
Cell Culture Supernates (n=5)11086-120
Heparin Plasma (n=6)10181-115
Serum (n=5)9984-113
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-6 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse IL-6 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-6
IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.
  • Long Name
    Interleukin 6
  • Entrez Gene IDs
    3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit);
  • Alternate Names
    B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A;
Related Research Areas
  • Adipocytokines

  • Cancer Biomarkers

  • Conventional/Classical Dendritic Cells

  • Cytokines and Receptors in Allergy and Asthma

  • Cytokines and Receptors in Angiogenesis

  • Cytokines and Receptors in Tfh Cell Differentiation and Function

  • Diabetes

  • gamma delta T Cells

  • Glucose Homeostasis

  • Helper T (Th) Cells

  • HIF Transcription Factors

  • IL-6 Family

  • Infectious Diseases

  • Inflammatory Mediators

  • Macrophage Activation Markers

  • Molecules Secreted by VSMC

  • Multiple Myeloma

  • Multiple Sclerosis and Myelin Related Molecules

  • Myeloid-derived Suppressor Cells (MDSC)

  • Neuroinflammation

  • Obesity

  • Plasmacytoid Dendritic Cells

  • T Cell Cytokine Signaling

  • Th17 Cells

  • Th22 Cells

  • Th9 Cells




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