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现在位置首页>实验试剂制品>生化试剂>ELISA试剂盒> Assay Designs Hsp70高灵敏度检测ELISA Kit

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Assay Designs Hsp70高灵敏度检测ELISA Kit

价    格:询价

产    地:美国更新时间:2019/7/15 13:41:34

品    牌:Assay Designs型    号:

状    态:正常点击量:3156

18253171090
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济南佰源医疗大发3d有限公司

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 Hsp70是一种分子伴侣,当细胞或有机体暴露于应激情况下可诱导其表达。它阻止蛋白质聚集,促进因受环境刺激、病原及疾病损伤的蛋白质的重新折叠。Hsp70的活性对于应激情况下的细胞存活和复员、以及对于在非应激情况下正常细胞功能的维持都是必需的。

    Hsp70是衰老、行经、体力活动如锻炼身体、精子发生等许多正常生理学过程的一个重要调节因子;Hsp70 在多种疾病和生理学过程中发挥作用,包括细胞凋亡、自身免疫、癌症、高血压、高热、缺氧、感染 (细菌和病毒)、炎症、局部缺血、器官移植以及暴露于化学制剂的中毒。 

2008年2月,Assay Designs™ 全新推出一种即用型酶免疫试剂盒用于检测血清和血浆中的Hsp70。

 特点和优点

          样本类型:目前市场上仅有的用于检测血清和血浆样本Hsp70的ELISA

          灵敏:检测低至90 pg/mL Hsp70

          经过验证:已检测了应激和非应激的血清和血浆样本

          特异性:与其他Hsp70家族成员的反应性低

 相关产品

         Hsp70 EIA Kit, Cat.# EKS-700B

         Hsp70 (anti-human IgG/A/M) ELISA Kit, Cat.# EKS-750

         Hsp70B’ EIA Kit, Cat.# EKS-725

 Hsp70试剂盒产品比较

 

参数

Hsp70高灵敏度EIA Kit

Hsp70 EIA Kit

目录号

EKS-715

EKS-700B

标准品范围

0.20 – 12.5 ng/mL

0.78 – 50 ng/mL

灵敏度

90 pg/mL

200 pg/mL

检测用时

4.5小时

4.5小时

孵育条件

室温

室温

标准品

人重组Hsp70

人重组Hsp70

贮存

4℃,标准品于-20℃

4℃,标准品于-20℃

捕获抗体

单克隆抗体

单克隆抗体

检测抗体

多克隆抗体

多克隆抗体

样本类型

血清和EDTA抗凝血浆

细胞裂解物和组织抽提物

种属反应性

人,小鼠,大鼠

人,小鼠,大鼠

每个试剂盒可检测的样本数

复孔39个

复孔40个

测定间精密度

12.8 – 19.1 %CV

12.0 – 13.8 %CV

测定内精密度

3.9 – 11.4 %CV

3.2 – 4.9 %CV

 常见问题与解答

Ÿ     Assay Designs会停产Hsp70 ELISA, EKS-700B吗?

不会。Assay Designs会继续供应EKS-700B用于细胞裂解物和组织抽提物样本。一般来说,检测这些样本不需要额外的灵敏度,因为细胞裂解物和组织能以Western blot检测(灵敏度低于EKS-700B)。血清和血浆样本则需要更高的灵敏度。

Ÿ     Hsp70 High Sensitivity EIA Kit, EKS-715, 适用于其他样本类型吗?

EKS-7, , , , 15目前只经验证可以用于血清和血浆。尽管该试剂盒可读取细胞裂解物样本,但尚需要进一步优化检测以达到与EKS-700B更接近的水平。

Ÿ     在EKS-715中可以使用枸橼酸盐或肝素抗凝血清吗?

Assay Designs及其beta测试者广泛验证了EDTA血浆样本。肝素血浆已被证明具有平行性,更多数据正待收集。但此刻我们还不能得出结论说肝素和枸橼酸盐是否能用于此试剂盒。

Ÿ     EKS-715可以检测其他种属的样本吗?

与人类Hsp70有高序列同源性的、从其他种属来源的Hsp70可能也可以被检测。但是Assay Designs只评估了人、小鼠和大鼠。.

Ÿ     以EKS-715和以EKS-700检测血清/血浆的Hsp水平会得到相同的结果吗?

从beta测试者处得到的反馈说,血清和血浆的比率似乎是一样的。有些测试者观察到用EKS-715测得的水平略微低于EKS-700的。有些则能在血清样本中检测到以前用EKS-700检测不到的Hsp70。


产品参数

Enzo(Assay Designs)NFκB,p65高灵敏度ELISA Kit
 

Enzo 旗下Assay Designs NFκB,p65 ELISA Kit提供了一种灵敏、快速、可靠的检测活化形式的NFκB,p65的1方法。这个方法使用亲和素包被的酶标板以及生物素标记的NFκB 抗体只捕获活化形式的NFκB。被捕获的NFκB被特异性NFκB p65-HRP抗体检测。该试剂盒用化学发光Elisa法检测,有TNF a Activated HeLa Cell Nuclear Extract做阳性对照,有Wild Type NFκB Competitor Duplex用于封闭以降低非特异性。

NFκB 是序列特异性转录激活剂,在B淋巴细胞中与k轻链基因的增强子结合。NFκB是一个异二聚体,由50 KD的DNA结合亚基(p50)和65 KD的转活亚基(p65/RelA)组成。p50/ p65异二聚体在胞液中与抑制子IκB结合,以非活化的状态存在。在一系列刺激物如LPS、前-炎症细胞因子和病毒感染等的刺激下,IκB被磷酸化,然后被蛋白小体降解。激活的NFκB异二聚体转入细胞核,诱导基因表达。 

特点:

  • 快速 - 3个小时检测活性NFκB p65;
  • 灵敏 - 化学发光检测灵敏于比色法;
  • 方便 - 提供足够2 x 96 wells的试剂;
  • 高通量 – 基于96孔板的方法提供了比EMSA 和 Western blot更高的通量;
  • 特异- 与传统Elisa相比,具有更好的信噪比和更少的非特异性结合。

检测种属:人、小鼠、大鼠

样本类型:细胞裂解液(Cell lylate 

检测范围0.01-10 ug

 

 

订购信息:

ADI-EKS-446 2x96 well NFκB p65 ELISA kit 

 

 

 

 

上图显示了2年8个批号的PGE2 EIA kit Cat. # ADI-900-001 的标准曲线。显示了高度的批间稳定性。

详细产品列表:

货号

产品名称

规格

ADI-900-071

Cortisol EIA kit

96 wells

ADI-900-066

Direct cAMP EIA kit

96 wells

ADI-900-014

Direct cGMP EIA kit

96 wells

ADI-900-001

PGE2 EIA kit

96 wells

ADI-901-001

PGE2 EIA kit

5 x 96 wells

ADI-900-002

TXB2 EIA kit

96 wells

ADI-901-002

TXB2 EIA kit

5 x 96 wells

ADI-930-001

PGE2 high sensitivity EIA kit

96 wells

ADI-900-097

Corticosterone EIA kit

96 wells

ADI-900-017

Arg8-Vasopressin EIA kit

96 wells

ADI-900-153

Oxytocin EIA kit

96 wells

ADI-900-057

Protein A EIA kit

96 wells

ADI-900-135

β-Catenin EIA kit

96 wells

ADI-900-151

Dkk-1 (human), EIA kit

96 wells

ADI-900-020A

Endothelin-1 EIA kit

96 wells

ADI-EKS-700B

HSP70 EIA kit

96 wells

ADI-EKS-750

Anti-HSP70 IgG/A/M (human), ELISA kit

96 wells

ADI-EKS-800

HO-1 (human), EIA kit

96 wells

ADI-EKS-715

HSP70 High Sensitivty EIA kit

96 wells

ADI-EKS-895

HSP90α (human), EIA kit

96 wells

ADI-EKS-350

DNA Damage EIA kit

96 wells

ADI-901-097

Corticosterone EIA kit

5 x 96 wells

ADI-901-071

Cortisol EIA kit

5 x 96 wells

ADI-901-066

Direct cAMP EIA kit

5 x 96 wells

ADI-901-163

cAMP Complete EIA kit

5 x 96 wells

ADI-901-014

Direct cGMP EIA kit

5 x 96 wells

ADI-901-153

Oxytocin EIA kit

5 x 96 wells

ADI-931-001

PGE2 high sensitivity EIA kit

5 x 96 wells

ADI-900-152

Erk1/2 EIA kit

96 wells

ADI-900-144

GSK-3β EIA kit

96 wells

ADI-900-122A

MEK1 EIA kit

96 wells

ADI-900-139

p27/Kip1 (human), EIA kit

96 wells

ADI-900-170

[pSer15]HSP27 (human), EIA kit

96 wells

ADI-900-119

[pSer218/Ser222]MEK1 EIA kit

96 wells

ADI-900-162

[pSer473]Akt1/2 EIA kit

96 wells

ADI-900-165

[pSer78]HSP27 (human), EIA kit

96 wells

ADI-900-123A

[pSer9]GSK-3β EIA kit

96 wells

ADI-900-101

[pThr180/Tyr182]p38 EIA kit

96 wells

ADI-900-106

[pThr183/Tyr185]Jnk1/2 EIA kit

96 wells

ADI-910-098

[pThr202/Tyr204]Erk1/2 CLIA kit

96 wells

ADI-900-098

[pThr202/Tyr204]Erk1/2 EIA kit

96 wells

ADI-900-092

11-dehydro-TXB2 EIA kit

96 wells

ADI-901-092

11-dehydro-TXB2 EIA kit

5 x 96 wells

ADI-900-050

12(S)-HETE EIA kit

96 wells

ADI-901-050

12(S)-HETE EIA kit

5 x 96 wells

ADI-900-108

13(S)-HODE EIA kit

96 wells

ADI-901-108

13(S)-HODE EIA kit

5 x 96 wells

ADI-900-051

15(S)-HETE EIA kit

96 wells

ADI-901-051

15(S)-HETE EIA kit

5 x 96 wells

ADI-900-023

15-deoxy-Δ12,14-PGJ2 EIA kit

96 wells

ADI-901-023

15-deoxy-Δ12,14-PGJ2 EIA kit

5 x 96 wells

ADI-900-174

17β-Estradiol (serum/plasma) EIA kit

96 wells

ADI-901-174

17β-Estradiol (serum/plasma) EIA kit

5 x 96 wells

ADI-900-008

17β-Estradiol EIA kit

96 wells

ADI-901-008

17β-Estradiol EIA kit

5 x 96 wells

ADI-900-004

6-keto-PGF1α EIA kit

96 wells

ADI-901-004

6-keto-PGF1α EIA kit

5 x 96 wells

ADI-900-010

8-iso-PGF2α EIA kit

96 wells

ADI-901-010

8-iso-PGF2α EIA kit

5 x 96 wells

ADI-900-012

9α,11β-PGF2α EIA kit

96 wells

ADI-901-012

9α,11β-PGF2α EIA kit

5 x 96 wells

ADI-907-026

Acetyltransferase activity kit

96 wells

ADI-EKS-400A

Akt Kinase activity kit

96 wells

ADI-900-173

Aldosterone EIA kit

96 wells

ADI-901-173

Aldosterone EIA kit

5 x 96 wells

ADI-ADK-700-E

Annexin V-FITC kit

100 mg

ADI-ADK-700-F

Annexin V-FITC kit

200 mg

ADI-EKS-650

Anti-HSP60 IgG/A/M (human), ELISA kit

96 wells

ADI-901-017

Arg8-Vasopressin EIA kit

5 x 96 wells

ADI-900-138

Bax (human), EIA kit

96 wells

ADI-900-133

Bcl-2 (human), EIA kit

96 wells

ADI-900-022

Big Endothelin-1 (human), EIA kit

96 wells

ADI-900-073

Big Endothelin-1 (rat), EIA kit

96 wells

ADI-900-163

cAMP Complete EIA kit

96 wells

ADI-900-067

cAMP EIA kit

96 wells

ADI-901-067

cAMP EIA kit

5 x 96 wells

ADI-907-013

Caspase-3 colorimetric detection kit

96 wells

ADI-907-014

Caspase-3 fluorometric detection kit

96 wells

ADI-907-027

Catalase fluorometric detection kit

500 tests

ADI-900-164

cGMP Complete EIA kit

96 wells

ADI-901-164

cGMP Complete EIA kit

5 x 96 wells

ADI-900-013

cGMP EIA kit

96 wells

ADI-901-013

cGMP EIA kit

5 x 96 wells

ADI-907-001

Chemiluminescent Labeling kit

5 x 1mg reactions

ADI-900-166

Comet SCGE Assay kit

50 tests

ADI-900-058

Complement C3a des Arg (human), EIA kit

96 wells

ADI-901-058

Complement C3a, des Arg (human), EIA kit

5 x 96 wells

ADI-900-059

Complement C4a des Arg (human), EIA kit

96 wells

ADI-901-059

Complement C4a des Arg (human), EIA kit

5 x 96 wells

ADI-920-071

Cortisol FPIA kit

100 tests

ADI-907-003

COX activity kit

96 wells

ADI-900-094

COX-2 (human), EIA kit

96 wells

ADI-907-035

Creatinine (serum) detection kit

2 x 96 wells

ADI-907-030

Creatinine colorimetric detection kit

2 x 96 wells

ADI-937-001

Creatinine colorimetric detection kit

10 x 96 wells

ADI-900-070

Cysteinyl Leukotriene EIA kit

96 wells

ADI-901-070

Cysteinyl Leukotriene EIA kit

5 x 96 wells

ADI-900-141

Cytochrome c (human), EIA kit

96 wells

ADI-900-093

DHEA EIA kit

96 wells

ADI-901-093

DHEA EIA kit

5 x 96 wells

ADI-900-091

Direct 8-iso-PGF2α EIA kit

96 wells

ADI-901-091

Direct 8-iso-PGF2α EIA kit

5 x 96 wells

ADI-900-172

Dkk-1 (mouse), EIA kit

96 wells

ADI-900-171

Dkk-1 (rat), EIA kit

96 wells

ADI-900-100

Estriol EIA kit

96 wells

ADI-907-031

Formaldehyde fluorometric detection kit

96 wells

ADI-900-026

Gastrin I (human), EIA kit

96 wells

ADI-901-026

Gastrin I (human), EIA kit

5 x 96 wells

ADI-900-149

Gastrin I (rat), EIA kit

96 wells

ADI-900-160

Glutathione (total), detection kit

4 x 96 wells

ADI-907-037

Glutathione fluorescent detection kit

96 wells

ADI-927-001

Glutathione fluorescent detection kit

5 x 96 wells

ADI-900-158

Glutathione Peroxidase activity kit

5 x 96 wells

ADI-900-159

Glutathione Reductase activity kit

5 x 96 wells

ADI-907-039

Glutathione Reductase fluorescent detection kit

96 wells

ADI-907-033

Glutathione S-Transferase fluorescent activity kit

96 wells

ADI-900-074

GRO/CINC-1 (rat), EIA kit

96 wells

ADI-907-034

Hemoglobin detection kit

2 x 96 wells

ADI-EKS-810A

HO-1 (rat), EIA kit

96 wells

ADI-EKS-500

HSP27 (human), EIA kit

96 wells

ADI-EKS-600

HSP60 (human), EIA kit

96 wells

ADI-EKS-725

HSP70B'' EIA kit

96 wells

ADI-907-012

Hydrogen Peroxide chemiluminescent detection kit

96 wells

ADI-907-015

Hydrogen Peroxide colorimetric detection kit

96 wells

ADI-907-028

Hydrogen Peroxide fluorometric detection kit

500 tests

ADI-900-136

IFN-γ (human), EIA kit

96 wells

ADI-900-137

IFN-γ (mouse), EIA kit

96 wells

ADI-900-150

IGF-1 (human), EIA kit

96 wells

ADI-900-109

IgG1 (mouse), EIA kit

96 wells

ADI-900-113

IgG2a (mouse), EIA kit

96 wells

ADI-900-110

IgG2b (mouse), EIA kit

96 wells

ADI-900-121

IgG3 (mouse), EIA kit

96 wells

ADI-900-120

IgM (mouse), EIA kit

96 wells

ADI-900-036

IL-10 (human), EIA kit

96 wells

ADI-901-036

IL-10 (human), EIA kit

5 x 96 wells

ADI-902-036

IL-10 (human), EIA kit

10 x 96 wells

ADI-900-148

IL-10 (mouse), EIA kit

96 wells

ADI-900-130

IL-1β (human), EIA kit

96 wells

ADI-900-132

IL-1β (mouse), EIA kit

96 wells

ADI-900-131

IL-1β (rat), EIA kit

96 wells

ADI-900-118A

IL-2 (human), EIA kit

96 wells

ADI-901-118

IL-2 (human), EIA kit

5 x 96 wells

ADI-902-118

IL-2 (human), EIA kit

10 x 96 wells

ADI-900-042

IL-2 (mouse), EIA kit

96 wells

ADI-900-145

IL-4 (human), EIA kit

96 wells

ADI-900-043

IL-4 (mouse), EIA kit

96 wells

ADI-900-033

IL-6 (human), EIA kit

96 wells

ADI-901-033

IL-6 (human), EIA kit

5 x 96 wells

ADI-902-033

IL-6 (human), EIA kit

10 x 96 wells

ADI-900-045

IL-6 (mouse), EIA kit

96 wells

ADI-900-156

IL-8 (human), EIA kit

96 wells

ADI-901-156

IL-8 (human), EIA kit

5 x 96 wells

ADI-902-156

IL-8 (human), EIA kit

10 x 96 wells

ADI-960-143

ImmunoSet™ Grp75 ELISA development set

5 x 96 wells

ADI-960-077

ImmunoSet™ Grp94 ELISA development set

5 x 96 wells

ADI-960-800

ImmunoSet™ HO-1 (human), ELISA development set

5 x 96 wells

ADI-960-071

ImmunoSet™ HO-1 (mouse), ELISA development set

5 x 96 wells

ADI-960-810

ImmunoSet™ HO-1 (rat), ELISA development set

5 x 96 wells

ADI-960-075

ImmunoSet™ HSP25 (rodent), ELISA development set

5 x 96 wells

ADI-960-076

ImmunoSet™ HSP27 high sensitivity (human), ELISA development set

5 x 96 wells

ADI-960-073

ImmunoSet™ HSP40 (human), ELISA development set

5 x 96 wells

ADI-960-142

ImmunoSet™ Osteopontin (human), ELISA development set

10 x 96 wells

ADI-960-070

ImmunoSet™ p53/MDM2 Complex ELISA development set

5 x 96 wells

ADI-960-072

ImmunoSet™ PDI ELISA development set

5 x 96 wells

ADI-960-074

ImmunoSet™ αB-Crystallin ELISA development set

5 x 96 wells

ADI-900-028A

Leptin (human), EIA kit

96 wells

ADI-900-019A

Leptin (mouse), EIA kit

96 wells

ADI-900-015A

Leptin (rat), EIA kit

96 wells

ADI-900-068

LTB4 EIA kit

96 wells

ADI-901-068

LTB4 EIA kit

5 x 96 wells

ADI-900-077

MCP-1 (rat), EIA kit

96 wells

ADI-907-025

Methyltransferase activity kit

96 wells

ADI-907-032

Methyltransferase HT activity kit

96 wells

ADI-900-115

Myeloperoxidase (human), EIA kit

96 wells

ADI-907-029

Myeloperoxidase fluorometric detection kit

500 tests

ADI-EKS-446

NFκB p65 ELISA kit

2 x 96 wells

ADI-917-010

Nitric Oxide (NO2/NO3) detection kit

2 x 96 wells

ADI-917-020

Nitric Oxide (total), detection kit

2 x 96 wells

ADI-900-142

Osteopontin (human), EIA kit

96 wells

ADI-900-090A

Osteopontin (rodent), EIA kit

96 wells

ADI-900-161

p21 (human), EIA kit

96 wells

ADI-907-041

p450 fluorescent activity kit

96 wells

ADI-900-117

p53 EIA kit

96 wells

ADI-EKS-470

p70 S6K activity kit

96 wells

ADI-907-038

Palladium detection kit

96 wells

ADI-900-005

PGE1 EIA kit

96 wells

ADI-901-005

PGE1 EIA kit

5 x 96 wells

ADI-910-001

PGE2 CLIA kit

96 wells

ADI-920-001

PGE2 FPIA kit

100 tests

ADI-900-069

PGF2α EIA kit

96 wells

ADI-901-069

PGF2α EIA kit

5 x 96 wells

ADI-930-069

PGF2α high sensitivity EIA kit

96 wells

ADI-931-069

PGF2α high sensitivity EIA kit

5 x 96 wells

ADI-900-146

Pin1 EIA kit

96 wells

ADI-EKS-390A

PKA Kinase activity kit

96 wells

ADI-EKS-420A

PKC Kinase activity kit

96 wells

ADI-900-011

Progesterone EIA kit

96 wells

ADI-901-011

Progesterone EIA kit

5 x 96 wells

ADI-EKS-450

Rac1 activation kit

30 tests

ADI-EKS-455

Rap1 activation kit

30 tests

ADI-EKS-460

Ras activation kit

30 tests

ADI-EKS-465

Rho activation kit

30 tests

ADI-900-175

Serotonin EIA kit

96 wells

ADI-900-125

Smac/DIABLO (human), EIA kit

96 wells

ADI-900-157

SOD activity kit

5 x 96 wells

ADI-907-002

sPLA2 activity kit

96 wells

ADI-900-018

Substance P EIA kit

96 wells

ADI-901-018

Substance P EIA kit

5 x 96 wells

ADI-900-111

Survivin (human), EIA kit

96 wells

ADI-900-065

Testosterone EIA kit

96 wells

ADI-901-065

Testosterone EIA kit

5 x 96 wells

ADI-900-155

TGF-β1 EIA kit

96 wells

ADI-907-036

Thiol fluorescent detection kit

96 wells

ADI-900-099

TNF-α (human), EIA kit

96 wells

ADI-901-099

TNF-α (human), EIA kit

5 x 96 wells

ADI-902-099

TNF-α (human), EIA kit

10 x 96 wells

ADI-900-047

TNF-α (mouse), EIA kit

96 wells

ADI-900-086A

TNF-α (rat), EIA kit

96 wells

ADI-900-025

Urinary Prostacyclin EIA kit

96 wells

ADI-901-025

Urinary Prostacyclin EIA kit

5 x 96 wells

ADI-900-080

VEGF (human), EIA kit

96 wells

ADI-900-124

XIAP (human), EIA kit

96 wells




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