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Der f 1 ELISA kit (EL-DF1)/尘螨过敏原

价    格:询价

产    地:美国更新时间:2019/7/15 13:41:34

品    牌:Indoor Biotechnologies型    号:kit

状    态:正常点击量:668

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上海博升生物科技有限公司

联 系 人:管小姐

电     话:021-54253791 / 400-670-5918

传     真:021-64280007

等     级: (第 10年)

性     质:生产型,贸易型,

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Der f 1 ELISA kit (EL-DF1)/尘螨过敏原

Assay   Performance:

Intra-assay   CV% of 7.1; Inter-assay CV% of 15

Contents:

Monoclonal   antibody 6A8, Universal Allergen Standard, Biotinylated antibody 4C1;   sufficient reagents for 10 plates.

Storage:

Store   at 4°C.

Not   Provided:

50mM   Carbonate/Bicarbonate buffer, pH 9.6
  Phosphate Buffered Saline, pH 7.4, containing 0.05% Tween 20 (PBS-T)
  Bovine Serum Albumin
  Streptavidin-Peroxidase
  1mM ABTS in 70mM citrate-phosphate buffer, pH 4.2

For research and commercial use in vitro: not for human in vivo or therapeutic use.



产品参数

Der f 1 ELISA kit (EL-DF1)/尘螨过敏原

 

Certificate of Analysis

Monoclonal   Antibody:

6A8   (clone 6A8 B10 D12)

Vial:

Red   Cap; 100μl

Immunogen:

Der   f 1

Isotype:

Mouse   IgG1

Specificity:

Binds   to a species specific epitope present on Dermatophagoides farinae allergen,   Der f 1.

Purification:

Produced   in tissue culture and purified by affinity chromatography using Protein A.   Single heavy and light chain bands on SDS-PAGE.

Concentration:

1mg/ml   in phosphate buffered saline, pH 7.4. Based on A280 for IgG (1.42=1mg/ml),   0.22μm filtered, preservative free.

 

Certificate of Analysis

Monoclonal   Antibody:

4C1   (clone 4C1 B8 3F8)

Vial:

Brown   Cap; 100μl

Immunogen:

Der   f 1

Isotype:

Mouse   IgG1

Specificity:

Binds   to a common epitope present on mite Dermatophagoides Group 1 allergens (Der f   1, Der p 1, Der m 1, Eur m 1).

Purification:

Produced   in ascites and purified by affinity chromatography using Protein A. Single   heavy and light chain bands on SDS-PAGE.

Biotinylation:

Biotinylated   and titrated for use in ELISA at 1/1000 dilution. Prepared in 1% BSA/50%   glycerol/PBS, pH 7.4, 0.22μm filtered, preservative free.

 

Certificate of Analysis

Allergen   Standard:

Universal   Allergen Standard

Vial:

White   Cap; 400μl

Composition:

A   formulation of eight purified natural allergens prepared in 1% BSA/50%   glycerol/PBS, pH 7.4

Concentration/Calibration:

 

Universal   Allergen Standard

Protein   Measurement

Concentration (ng/ml)

Der   p 1

Amino-acid   analysis

2500

Der f 1

Amino-acid analysis

2500

Der   p 2

Amino-acid   analysis

1000

Fel   d 1

Amino-acid   analysis

1000

Can   f 1

Amino-acid   analysis

2500

Rat   n 1

Amino-acid   analysis

1000

Mus   m 1

Amino-acid   analysis

250

Bla   g 2

Amino-acid   analysis

2500

References:

1.     Chapman, M.D., Sutherland, W.M., and Platts-Mills, T.A.E. (1984).    Recognition of two Dermatophagoides pteronyssinus specific epitopes on   Antigen P1 using  monoclonal antibodies:  Binding to each epitope   can be inhibited be sera from mite allergic patients.  J. Immunol.   133:2488-2495.

2.    Ford, A.W., Rawle, F.C., Lind, P., Spieksma, F.Th.M., Lowenstein, H. and   Platts-Mills, T.A.E. (1985). Standardization of Dermatophagoides   pteronyssinus: Assessment of potency and allergen content in ten coded   extracts. Int. Archs. Allergy Appl. Immunol. 76:58-68.

3.    Heymann, P.W., Chapman, M.D. and Platts-Mills, T.A.E. (1986). Antigen Der f 1   from the dust mite Dermatophagoides farinae: Structural comparison with Der p   1 from D. pteronyssinus and epitope specificity of murine IgG and human IgE   antibodies. J. Immunol. 137:2841-2847.

4.     Chapman, M.D., Heyman, P.W., Wilkins, S.R., Brown, M.J., and Platts-Mills,   T.A.E. (1987). Monoclonal immunoassays for major dust mite (Dermatophagoides)   allergens, Der p 1 and Der f 1, and quantitative analysis of the allergen   content of mite and house dust extracts. J. Allergy Clin. Immunol.   80:184-194.

5.    Platts-Mills, T.A.E. and Chapman, M.D. (1987). Dust mites: Immunology,   allergic disease and environmental control. J. Allergy Clin. Immunol.   80:755-775

6.    Chapman, M.D., Heymann, P.W., and Platts-Mills, T.A.E. (1987). Epitope   mapping of two major inhalant allergens, Der p 1 and Der f 1, from mites of   the genus Dermatophagoides. J. Immunol. 139: 1479-1484.

7.    Chapman, M.D. (1988). Allergen specific monoclonal antibodies: New tools for   the management of allerigic disease. Allergy 43 (5):7-14.

8.    Luczynska, C.M., Arruda, L.K., Platts-Mills, T.A.E., Miller, J.D., Lopez, M.,   and Chapman, M.D. (1989). A two-site monoclonal antibody ELISA for the   quantification of the major Dermatophagoides spp. allergens, Der p 1 and Der   f 1. J. Immunol. Meths. 118:227-235.

9.    Platts-Mills T.A.E., Thomas W.R., Aalberse R.C., Vervloet D. and Chapman M.D.   (1992). Dust mite allergens and asthma: Report of a second international   workshop. J Allergy Clin Immunol 89:1046-1060.

10.    Platts-Mills T.A.E., Vervloet D., Thomas W.R., Aalberse R.C., Chapman M.D.   Indoor Allergens and Asthma: Report of the third international workshop. J   Allergy Clin Immunol, 1997; 100:S1-S24.

(For   complete reference list see EL-DF1 Certificate of Analysis)



产品介绍

Der f 1 ELISA kit (EL-DF1)/尘螨过敏原

INDOOR Biotechnologies Inc.(INDOOR)公司是一家专业开发生产用于研究哮喘以及其他过敏性疾病的免疫诊断和相关生物学检测产品的生物大发3d公司。公司在过敏原环境暴露水平的评估方面为世界领先。对过敏原结构,分子生物学和免疫反应的研究,公司处于世界前列。INDOOR公司致力于环境科学,过敏和哮喘相关的专业产品与服务,以供研究人员和消费者使用,通过研究、培训和开发尖端科技,为我们的客户和全球客户服务,以改善病人护理。INDOOR为政府及学术研究人员,全球500强的企业,室内空气质量的企业、过敏疫苗生产商和生物制药企业提供服务。 
       INDOOR生产的一系列过敏原研究和检测用抗体拥有来自美国弗吉尼亚大学的独家专利,覆盖范围广,包括屋尘螨、猫、狗、蟑螂、真菌和食物过敏原。这些抗体是包括ELISA和MARIA? 大发3d在内的过敏原检测和我们的Ventia? 快速过敏原检测实验分析平台的核心组分。IB的过敏原检测范围非常广泛,可用于对过敏原环境暴露水平的评估,也能让用户对使用的过敏原隔离清除程序、相关产品和大发3d的有效性进行可靠的评估,还可应用于过敏原标准化以及相关产品的研究开发。 
       INDOOR 还开发出了天然和重组的过敏原产品线,可用于研究和开发过敏原诊断产品和疫苗。这个产品线目前涵盖50多种纯化的过敏原,具有很高纯度和生物活性。这个产品线还包括低内毒素的纯化LoTox?过敏原,可用于过敏原和哮喘的免疫研究。 
       INDOOR的大发3d和产品已经在全球范围内被广泛使用,相关的科学论文已在超过250种科学出版物上发表。除提供产品以外IB还提供过敏原分析服务、合同研究服务以及专业培训服务。 



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