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商品编号:72357
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thermofisher EL0014 T4 DNA 连接酶

价    格:询价

产    地:美国更新时间:2019/7/15 13:41:34

品    牌:飞世尔型    号:EL0014

状    态:正常点击量:584

13346181029
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杭州乐乾科学仪器有限公司

联 系 人:周欢欢

电     话:0571-86049923

传     真:571-86049231

等     级: (第 5年)

性     质:生产型,贸易型,

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thermofisher  EL0014  T4 DNA 连接酶 描述

Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

T4 DNA Ligase requires ATP as a cofactor.

Highlights

? Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
? Fast—sticky-end ligation is completed in 10 minutes at room temperature
? Supplied with PEG solution for efficient blunt-end ligation

Applications

? Cloning of restriction enzyme generated DNA fragments
? Cloning of PCR products
? Joining of double-stranded oligonucleotide linkers or adaptors to DNA
? Site-directed mutagenesis
? Amplified fragment length polymorphism (AFLP)
? Ligase-mediated RNA detection (see Reference 3)
? Nick repair in duplex DNA, RNA or DNA/RNA hybrids
? Self-circularization of linear DNA.

Includes

? T4 DNA Ligase
? 10X T4 DNA Ligase Buffer
? 50% PEG Solution

Notes

? Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.
? The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.
? Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture using spin columns or chloroform extraction. The extracted DNA can be further precipitated with ethanol.



产品参数

200 units


产品介绍

thermofisher  EL0014  T4 DNA 连接酶 描述

Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

T4 DNA Ligase requires ATP as a cofactor.

Highlights

? Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
? Fast—sticky-end ligation is completed in 10 minutes at room temperature
? Supplied with PEG solution for efficient blunt-end ligation

Applications

? Cloning of restriction enzyme generated DNA fragments
? Cloning of PCR products
? Joining of double-stranded oligonucleotide linkers or adaptors to DNA
? Site-directed mutagenesis
? Amplified fragment length polymorphism (AFLP)
? Ligase-mediated RNA detection (see Reference 3)
? Nick repair in duplex DNA, RNA or DNA/RNA hybrids
? Self-circularization of linear DNA.

Includes

? T4 DNA Ligase
? 10X T4 DNA Ligase Buffer
? 50% PEG Solution

Notes

? Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.
? The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.
? Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture using spin columns or chloroform extraction. The extracted DNA can be further precipitated with ethanol.




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