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现在位置首页>实验试剂制品>>限制性内切酶> thermofishe T4 DNA 连接酶 (5*1000 units)

商品编号:72358
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thermofishe T4 DNA 连接酶 (5*1000 units)

价    格:询价

产    地:美国更新时间:2019/7/15 13:41:34

品    牌:飞世尔型    号:EL0012

状    态:正常点击量:519

13346181029
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杭州乐乾科学仪器有限公司

联 系 人:周欢欢

电     话:0571-86049923

传     真:571-86049231

等     级: (第 5年)

性     质:生产型,贸易型,

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thermofishe T4 DNA 连接酶 (5*1000 units)描述

Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

T4 DNA Ligase requires ATP as a cofactor.

Highlights

? Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
? Fast—sticky-end ligation is completed in 10 minutes at room temperature
? Supplied with PEG solution for efficient blunt-end ligation

Applications

? Cloning of restriction enzyme generated DNA fragments
? Cloning of PCR products
? Joining of double-stranded oligonucleotide linkers or adaptors to DNA
? Site-directed mutagenesis
? Amplified fragment length polymorphism (AFLP)
? Ligase-mediated RNA detection (see Reference 3)
? Nick repair in duplex DNA, RNA or DNA/RNA hybrids
? Self-circularization of linear DNA.

Includes

? T4 DNA Ligase
? 10X T4 DNA Ligase Buffer
? 50% PEG Solution

Notes

? Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.
? The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.
? Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture using spin columns or chloroform extraction. The extracted DNA can be further precipitated with ethanol.



产品参数

5 x 1,000 units


产品介绍

thermofishe T4 DNA 连接酶 (5*1000 units)描述

Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

T4 DNA Ligase requires ATP as a cofactor.

Highlights

? Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
? Fast—sticky-end ligation is completed in 10 minutes at room temperature
? Supplied with PEG solution for efficient blunt-end ligation

Applications

? Cloning of restriction enzyme generated DNA fragments
? Cloning of PCR products
? Joining of double-stranded oligonucleotide linkers or adaptors to DNA
? Site-directed mutagenesis
? Amplified fragment length polymorphism (AFLP)
? Ligase-mediated RNA detection (see Reference 3)
? Nick repair in duplex DNA, RNA or DNA/RNA hybrids
? Self-circularization of linear DNA.

Includes

? T4 DNA Ligase
? 10X T4 DNA Ligase Buffer
? 50% PEG Solution

Notes

? Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.
? The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.
? Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture using spin columns or chloroform extraction. The extracted DNA can be further precipitated with ethanol.




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