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thermofisher FastDigest SmaI FD0663

价    格:询价

产    地:美国更新时间:2019/7/15 13:41:34

品    牌:飞世尔型    号:FD0663

状    态:正常点击量:800

13346181029
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杭州乐乾科学仪器有限公司

联 系 人:周欢欢

电     话:0571-86049923

传     真:571-86049231

等     级: (第 5年)

性     质:生产型,贸易型,

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thermofisher FastDigest SmaI  FD0663

Features

? 100% activity of all FastDigest enzymes in the universal buffer
? 100% buffer compatibility with downstream applications
? Complete digestion in 5–15 minutes
? Direct loading on gels
? No star activity
? 176 FastDigest enzymes available

Applications

? Molecular cloning
? Restriction site mapping
? Genotyping
? Southern Blot
? Restriction fragment length polymorphism (RFLP)
? SNP analysis

Note: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.

For methylation sensitivity, refer to product specifications.


产品参数

100 reactions


产品介绍

thermofisher FastDigest SmaI  FD0663

Thermo Scientific FastDigest SmaI restriction enzyme recognizes CCC^GGG site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: TspMI, XmaCI, XmaI.

Thermo Scientific FastDigest SmaI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.

The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow FragmentT4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.

For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.

Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.



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