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美国Stemgent 干细胞研究试剂

价    格:询价

产    地:美国更新时间:2019/7/15 13:41:34

品    牌:Stemgent型    号:

状    态:正常点击量:3022

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上海翊圣生物科技有限公司

联 系 人:翊圣生物

电     话:400-6111-883

传     真:021-34615995

等     级: (第 12年)

性     质:生产型,贸易型,服务型,

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Stemgent公司有IPS细胞和ES细胞等干细胞研究试剂可供选择,可以为全世界的干细胞研究人员提供开发、改良的具有良好重复性的产品!
Small Molecules From Stemgent

Be they natural metabolites or synthetic low molecular weight organic compounds, small molecules demonstrate diverse biological function. Their impact on cell signaling pathways and downstream effects on normal and abnormal cell growth and differentiation is of intense interest to stem cell biologists. An expanding array of small molecules has been shown to modulate the fate of stem cells by either promoting differentiation and proliferation, or by maintaining them in a pluripotent, undifferentiated state.

Induced pluripotent stem (iPS) cell generation, a critical tool in stem cell research, has traditionally been achieved by viral transduction of oncogenic transcription factors – a challenging process. Recent studies, however, have shown that some of the four genes originally thought to be required for iPS cell generation may be dispensable. Several alternative defined conditions are capable of improving efficiency and kinetics, generating more homogeneous iPS populations. These defined conditions are made possible with the use of various combinations of small molecules affecting reprogramming, self-renewal, and differentiation.

Stemolecules™ are of the highest purity (≥98%), structurally verified by NMR and mass spectrometry, and tested for cytotoxicity – and many are exclusive to Stemgent.
 
Small Molecules
Stemolecule™ Thiazovivin , Cat. No. 04-0017
Stemolecule™ Thiazovivin dramatically improves the survival of hESCs upon trypsinization1. Thiazovivin in combination with inhibitors of the TGFβ receptor and the MEK pathway improve reprogramming efficiency more than 200-fold1.
Stemolecule™ Rock II Inhibitor , Cat. No. 04-0030
StemoleculeTM Rock II Inhibitor is a specific inhibitor for type 2 Rho-associated, coiled-coil containing protein kinase (Rock). Rock inhibitors have been found to prevent apoptosis, as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency.
Stemolecule™ Rock I Inhibitor , Cat. No. 04-0029
Rock inhibitors, including Stemolecule™ Rock I Inhibitor, have been found to prevent apoptosis, as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency.
Stemolecule™ Cyclic Pifithrin-α , Cat. No. 04-0040
Stemolecule™ Cyclic Pifithrin-α (Cyclic PFT-α) is a transcriptional inhibitor of p53. The reduction of p53 activity has been shown to enhance reprogramming efficiency of human and mouse somatic cells. Cyclic PFT-α sensitizes tumor cells to antimicrotubule agent induced apoptosis.
Stemolecule™ Pifithrin-α , Cat. No. 04-0038
Stemolecule™ Pifithrin-α (PFT-α) is a small molecule inhibitor of p53 dependent apoptosis and transcriptional activation for cyclin G, p21/waf1, and mdm2 expression. The reduction of p53 activity has been shown to enhance reprogramming efficiency of human and mouse somatic cells.
Stemolecule™ BMP Inhibitor LDN-193189 , Cat. No. 04-0019
StemoleculeTM BMP Inhibitor LDN-193189 functions primarily through inhibition of BMP type I receptors ALK2 and ALK3. LDN-193189 only weakly inhibits ALK4, ALK5, and ALK7. BMP signaling coordinates developmental patterning and has essential physiological roles in mature organisms.
Stemolecule™ (-)-Indolactam V , Cat. No. 04-0020
StemoleculeTM (-)-Indolactam V is a PKC isozyme inhibitor identified in a high-content chemical screen as a small molecule inducer of human embryonic stem cell differentiation to functional insulin-secreting beta cells at the definitive endoderm cell stage.
Stemolecule™ SHH Antagonist , Cat. No. 04-0032
StemoleculeTM SHH Antagonist is an inhibitor of Sonic hedgehog signaling (SHH). Hedgehog signaling is involved in embryogenesis as well as cancer progression. The mechanism for this antagonist is unknown.
Stemolecule™ SMO Antagonist , Cat. No. 04-0031
Stemolecule™ SMO Antagonist is an analog of SANT-2, an inhibitor of Sonic hedgehog signaling. Hedgehog signaling is involved in embryogenesis as well as cancer progression. The mechanism of action of SMO Antagonist is allosteric inhibition of Smoothened (SMO), an integral membrane protein mediator of Hedgehog signaling.
Stemolecule™ Wnt Inhibitor IWP-4 , Cat. No. 04-0036
Stemolecule™ Wnt Inhibitor IWP-4 is an antagonist of the Wnt/β-catenin pathway. Wnt Inhibitor IWP-4 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity1. It blocks phosporylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin.
Stemolecule™ Wnt Inhibitor IWP-3 , Cat. No. 04-0035
Stemolecule™ Wnt Inhibitor IWP-3 is an antagonist of the Wnt/β-catenin pathway. Wnt Inhibitor IWP-3 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity1. It blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin.
Stemolecule™ Wnt Inhibitor IWP-2 , Cat. No. 04-0034
Stemolecule™ Wnt Inhibitor IWP-2 is an antagonist of the Wnt/β-catenin pathway1. Wnt Inhibitor IWP-2 prevents palmitylation of Wnt proteins by Porcupine (Porcn), a membrane-bound O-acyltransferase, thereby blocking Wnt secretion and activity1. It blocks phosphorylation of the Lrp6 receptor and accumulation of both Dvl2 and β-catenin1.
Stemolecule™ IDE-2 , Cat. No. 04-0027
Stemolecule™ IDE-2 is an inducer of definitive endoderm (IDE) from embryonic stem (ES) cells in mouse and human ES cell cultures1. IDE-2 chemically induced endoderm can continue to differentiate into pancreatic progenitors in vitro and contribute to gut tube formation in vivo when injected into the developing gut tube of mouse embryos.
Stemolecule™ IDE-1 , Cat. No. 04-0026
Stemolecule™ IDE-1 is an inducer of definitive endoderm (IDE) from embryonic stem (ES) cells in mouse and human ES cell cultures. Endoderm chemically induced by IDE-1 can continue to differentiate into pancreatic progenitors in vitro and contribute to gut tube formation in vivo when injected into the developing gut tube of mouse embryos.
Stemolecule™ Doxycycline hyclate , Cat. No. 04-0016
Stemolecule™ Doxycycline hyclate (Dox), a derivative of tetracycline, is used to induce expression of the Stemgent® iPSC Generation Dox Inducible products. In the iPSC Generation Dox Inducible protocol, addition of Dox to the culture medium of transduced cells induces the reverse tetracycline transactivator (rtTA) to bind to the Tet response element and results in the transcriptional activation for each of the factors implicated in the reprogramming process. Conversely, Dox can be removed from the medium to suppress transcriptional activation of the reprogramming factors.
Stemolecule™ Forskolin , Cat. No. 04-0025
Stemolecule™ Forskolin can potentiate neuron differentiation and can also stimulate adenylate cyclase activity and increase cyclic AMP. Protein kinase A (PKA) activity is dependent upon binding to cAMP and has been implicated in sonic hedgehog signaling.
Stemolecule™ Dorsomorphin , Cat. No. 04-0024
Dorsomorphin-2HCl is a potent inhibitor of AMP-activated protein kinase (AMPK) and bone morphogenic protein (BMP) signaling, functioning through inhibition of BMP type I receptors ALK2, ALK3 and ALK6 and thus blocks BMP-mediated SMAD1/5/8 phosphorylation.
Stemolecule™ Dexamethasone , Cat. No. 04-0023
Stemolecule™ Dexamethasone is a glucocorticoid receptor agonist known to affect inflammatory responses, cellular proliferation, and differentiation in target tissues.
Stemolecule™ Cyclopamine , Cat. No. 04-0022
Stemolecule™ Cyclopamine has been identified as a specific inhibitor of hedgehog singaling by direct binding to the heptahelical bundle of Smoothened. Hedgehog signaling is involved in embryogenesis as well as cancer progression. Cyclopamine has been utilized as a small molecule inducer of stem cell differentiation towards definitive endoderm pancreatic islet cells, as a modulator of cell proliferation, and as an anticancer drug.
Stemolecule™ All-Trans Retinoic Acid , Cat. No. 04-0021
Stemolecule™ All-Trans Retinoic Acid functions as a signaling molecule for various developmental pathways that control differentiation and proliferation. It has been used in various differentiation protocols, including B-cells, T cells and neurons, and implicated in specification of the embryonic anterior/posterior axis through Hox gene regulation.
Stemolecule™ KAAD-Cyclopamine , Cat. No. 04-0028
Stemolecule™ KAAD-Cyclopamine is a sonic hedgehog antagonist known to target Smoothened. Hedgehog signaling is involved in embryogenesis as well as cancer progression. KAAD-Cyclopamine has been utilized to halt the migration or proliferation of a variety of cancer cells.
iPSC Generation
Stemolecule™ Tranylcypromine hydrochloride , Cat. No. 04-0033
Stemolecule™ Tranylcypromine inhibits BHC110/LSD1, a histone H3 lysine 4 demethylation enzyme with close homology to monoamine oxidases. Treatment of P19 embryonal carcinoma cells with tranylcypromine resulted in global increase in H3K4 methylation as well as transcriptional derepression of the pluripotent stem cell marker Oct4.
Kits / Sets
Stemolecule™ MAPK Signaling Pathway Modulator Set , Cat. No. 00-0041
The Stemolecule™ MAPK Signaling Pathway Modulator Set consists of three cell-permeable small molecules: PD0325901, PD173074, and SC1 (Pluripotin), which can modulate the MAPK signaling cascade. PD0325901 is a MEK inhibitor; PD173074 is an ATP-competitive inhibitor of the FGF receptor, known to activate the MAPK/ERK cascade; and SC1 (Pluripotin) acts through dual inhibition of ERK 1 and Ras-GAP. Each small molecule is tested for purity, accurate mass and structure, and toxicity in mouse embryonic stem cells.
Stemolecule™ iPS Cell Enhancer Set , Cat. No. 00-0040
The Stemolecule™ iPS Cell Generation Enhancer Set is a proven set of small molecules designed to improve iPS cell generation efficiency. The set consists of four cell-permeable small molecules: RG108, BIX1294, Valproic Acid (VPA), and R(+)Bay K 8644.
Stemolecule™ Epigenetic Modifier Set , Cat. No. 00-0039
The Stemolecule™ Epigenetic Modifier Set is a complete set of small molecules for modulating epigenetic effects of DNA and histones. The set consists of four cell-permeable small molecules: BIX01294, Sodium Butryate, RG108, and Valproic Acid (VPA). BIX01294 is a selective inhibitor of G9a HMTase, Sodium Butryate is an inhibitor of histone deacetylases, RG108 is a DNA methyltransferase inhibitor, and VPA is an inhibitor of histone deacetylases. Each small molecule is tested for purity, accurate mass and structure, and toxicity in mouse embryonic stem cells.
Stemolecule™ TGF-beta Signaling Pathway Modulator Set , Cat. No. 00-0038
The Stemolecule™ TGF-β Small Molecule Set is an inclusive set of small molecules for regulating the transforming growth factor beta (TGF -β) signaling pathway. The set consists of three Stemolecules™, SB431542, A83-01, and ALK5 Inhibitor: activin receptor-like kinase (ALK) receptors that work as TGF-β. Each small molecule is tested for purity, accurate mass and structure, and toxicity in mouse embryonic stem cells.
Components
Stemolecule™ ALK5 Inhibitor , Cat. No. 04-0015
ALK5 Inhibitor is a potent small molecule that selectively inhibits the TGF-β signaling pathway.
Stemolecule™ A83-01 , Cat. No. 04-0014
A83-01 is a potent small molecule that selectively inhibits ALK4, ALK5, and ALK7 receptors. This small molecule has been used to generate rat and human iPS cells towards a mouse ES cell like self-renewal state.
Stemolecule™ BIO , Cat. No. 04-0003
BIO is the first pharmacological agent shown to maintain self-renewal in human and mouse embryonic stem cells.1
Stemolecule™ CHIR99021 , Cat. No. 04-0004
CHIR99021 is the most selective inhibitor of GSK3β.
Stemolecule™ Sodium Butyrate , Cat. No. 04-0005
In cultured mouse and human cells, sodium butyrate has been shown to inhibit both the mRNA and protein content of cyclin D1.1 Sodium butyrate is a known inhibitor of histone deacetylases.2
Stemolecule™ Purmorphamine , Cat. No. 04-0009
Stemolecule™ Purmorphamine promotes the differentiation of human and murine mesenchymal progenitor cells into osteoblasts.1,2 It is ideal for stem cell and cell signaling researchers using human embryonic stem cells to investigate differentiation with small molecules.
Stemolecule™ SB431542 , Cat. No. 04-0010
SB431542 has been shown to stimulate proliferation, differentiation, and sheet formation of endothelial cells derived from embryonic stem cells via ALK receptor inhibition.1,2
iPSC Generation Components
Stemolecule™ PD173074 , Cat. No. 04-0008
PD173074 inhibits the FGF signaling pathway.
Stemolecule™ Valproic Acid , Cat. No. 04-0007
VPA has been shown to enhance iPS generation in a three-factor transduction (Oct4, Klf4 and Sox2) without introduction of the c-Myc oncogene.1
Stemolecule™ PD0325901 , Cat. No. 04-0006
PD0325901 selectively binds and inhibits MEK, which may result in the inhibition of the phosphorelation and activation of MAPK/ERK.1,2
Stemolecule™ SC1 (Pluripotin) , Cat. No. 04-0011
SC1 (Pluripotin) is a high-quality, affordable small molecule LIF replacement for mouse embryonic (mES) cell self-renewal. SC1 maintains mES cells in an undifferentiated self-renewal state in the absence of LIF, feeder cells, or serum.
Stemolecule™ Y27632 , Cat. No. 04-0012
Y27632 is a Rho-associated kinase ROCK inhibitor that enhances survival and cloning efficiency of human embryonic stem cells.
Stemolecule™ R(+)Bay K 8644 , Cat. No. 04-0013
R(+)Bay K 8644 is a small molecule that enhances reprogramming efficiency when used with BIX01294.
Stemolecule™ BIX01294 , Cat. No. 04-0002
BIX01294 enhances reprogramming efficiency of neural progenitor cells to the same levels as when four transcription factors (Oct4, Klf4, Sox2 and c-Myc) were introduced to somatic cells.1,2
Stemolecule™ RG108 , Cat. No. 04-0001
Stemolecule™ RG108 is the highest purity preparation available, only from Stemgent. RG108 is a cell-permeable, specific DNA methyltransferase inhibitor that displays anti-proliferative properties with no detectable cytotoxic effects.1




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